2-bromo-3-nitrobenzoic acid was analyzed by liquid phase method
1. Apparatus and reagents:
High performance liquid chromatography (HPLC), analytical balance, methanol chromatographic purity
2. Operation method:
Accurately weigh about 0.1g of the product, add 1ml methanol for dilution, and shake well.
3. Analysis conditions:
Column: 5um, C18, 4.6 * 150mm
Protective column cn
Flow rate: 2.0ml/min
Wavelength: 214nm
Injection volume: 0.2ul
Time: 15min
Mobile phase:
A: 0.1% trifluoroacetic acid aqueous solution
B: 0.1% trifluoroacetic acid acetonitrile solution
Analytical method of 2-bromo-3-nitrobenzoic acid

4. Normalization of calculation area, 98%+